Analysis Note

Controlhuman lymphocytes

Application

Detect CD23 using this Milli-Mark Anti-CD23-FITC Antibody, clone MHM6 validated for use in FC.

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD23 is a 45 kDa type II membrane glycoprotein belonging to the C-type lectin family, and is identical to the low affinity IgE receptor (FceRII) (Kikutani, 1987; Aubry, 1987). CD23 is primarily expressed on B cells and monocytes including a strong expression on EBV-transformed B lymphoblasts (Sarfati, 1997; Sarfati, 1995). CD23 is also present on a large variety of other cells such as T cells, eosinophils, platelets, Langerhans cells, and a subset of thymic epithelial cells (Sarfati, 1995). However, CD23 is absent in a large number of normal non-lymphoid human tissues, such as, central nervous system, endocrine glands, gastrointestinal, respiratory, urinary, and female/male reproductive system, skin, bone, cartilage, connective tissue, muscles, peripheral nerves, vessels, mesothelium, synovium, placenta, and umbilical cord (Pallesen, 1987). Anti-CD23, MHM6, was included in the Second, Third, Fourth, and Fifth International Workshop and Conference on Human Leukocyte Differentiation Antigens, and studies by a number of laboratories confirmed its reactivity with CD23 (Sarfati, 1995).

Immunogen

EBV-transformed B-lymphoblastoid cells

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Quality

Evaluated by flow cytometry using human lymphocytes.

Specificity

Antibody recognizes Human CD23 antigen.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

45 kDa (Kikutani, 1987; Aubry, 1987)